python - 在python中解析包含multifasta BLAST结果的xml文件

Question

我正在尝试解析包含 multifasta BLAST 结果的 xml 文件 - 这是 link - 大小约为 400kB。程序应返回四个序列名称。每个下一个结果应该首先位于(包含最佳对齐)“< Iteration_iter-num > n < Iteration_iter-num/>”之后，其中 n = 1,2,3,...

像这样:

< Iteration_iter-num >1< /Iteration_iter-num >

****Alignment****
sequence: gi|171864|gb|AAC04946.1| Yal011wp [Saccharomyces cerevisiae]

< Iteration_iter-num >2< /Iteration_iter-num >

****Alignment****
sequence: gi|330443384|ref|NP_009392.2| 

< Iteration_iter-num >3< /Iteration_iter-num >

****Alignment****
sequence: gi|6319310|ref|NP_009393.1|

< Iteration_iter-num >4< /Iteration_iter-num >

****Alignment****
sequence: gi|6319312|ref|NP_009395.1|

但结果我的程序返回这个:

<Iteration_iter-num>1</Iteration_iter-num>
****Alignment****
sequence: gi|171864|gb|AAC04946.1| Yal011wp [Saccharomyces cerevisiae]

<Iteration_iter-num>2</Iteration_iter-num>
****Alignment****
sequence: gi|171864|gb|AAC04946.1| Yal011wp [Saccharomyces cerevisiae]

<Iteration_iter-num>3</Iteration_iter-num>
****Alignment****
sequence: gi|171864|gb|AAC04946.1| Yal011wp [Saccharomyces cerevisiae]

<Iteration_iter-num>4</Iteration_iter-num>
****Alignment****
sequence: gi|171864|gb|AAC04946.1| Yal011wp [Saccharomyces cerevisiae]

如何从此 xml 文件获取另一个 BLASTA 结果？

这是我的代码:

from Bio.Blast import NCBIXML
from bs4 import BeautifulSoup

result = open ("BLAST_left.xml", "r")
records = NCBIXML.parse(result)
item = next(records)

file = open("BLAST_left.xml")
page = file.read()
soup = BeautifulSoup(page, "xml")
num_xml_array = soup.find_all('Iteration_iter-num')
i = 0
for records in records:
    for itemm in num_xml_array:
        print (itemm)
        for alignment in item.alignments:
            for hsp in alignment.hsps:
                print("\n\n****Alignment****")
                print("sequence:", alignment.title)
            break
        itemm = num_xml_array[i+1]
    break

//我知道我的英语并不完美，但我真的不知道该怎么办，而且我没有人可以要求，所以我选择了你:)

Answer 1

我认为 Biopython 是解析 XML 的更好选择，无需使用 BeautifulSoup:

from Bio.Blast import NCBIXML


result = open("BLAST_left.xml", "r")
records = NCBIXML.parse(result)

for i, record in enumerate(records):
    for align in record.alignments:
        print("Iteration {}".format(i))
        print(align.hit_id)
        break  # Breaking here gives you only the best HSP.